Kostas Tokatlidis

• Professor (former)
• Affiliated faculty member and Group Leader at IMBB-FORTH in Heraklion, Crete, Greece.

office

IMBB, FORTH

telephone

+302810391136

e-mail

tokatlidis@materials.uoc.gr, tokatlid@imbb.forth.gr

Lab webpages

http://www.mitochondria.gr
http://www.imbb.forth.gr/personal_page/tokatlidis.html

Research Interests

Current research acitvities

• Intracellular protein targeting, sorting, and organelle biogenesis
• Oxidative protein folding and assembly to macromolecular machines
• Mitochondria biogenesis in health and disease, redox signalling
• Protein engineering and biotechnological production of pharmaceutical proteins
• Biomolecular motors - single molecule approaches
• Mitochondria nanomedicine, drug delivery to mitochondria

Short Curriculum Vitae

Born in 1966, Veria, Greece. B.Sc. (1988) Chemical engineering, Aristotle University of Thessaloniki. Master’s (1991) in Chemical/Biochemical engineering and protein biochemistry (jointly Institut Pasteur, Paris and University of Delaware USA, work on formation of inclusion bodies of overexpressed proteins when produced in large scale in cell factories, funded by Fulbright Fellowship). Ph.D (1993) Chemical/Biochemical engineering and protein biochemistry (jointly Institut Pasteur, Paris and University of Delaware USA, work on inclusion bodies of overexpressed proteins when produced in large scale in cell factories and mechanistic analysis of the formation of a large multienzyme complex involved in degradation of celulose, the cellulosome, funded by EU fellowship. Postodoctoral associate (1993-1994) Protein folding, ribosome-chaperone associations (Institut Pasteur with Prof. Michel Goldberg, funded by EU postdoc fellowship). Postdoctoral Associate (1994-1998) Protein import and mitochondria biogenesis (Biozentrum, University of Basel, with Prof. Jeff Schatz, funded by HFSPO, EMBO, Roche and Swiss National Foundation postdoc fellowships). Establishement of independent group, Lecturer, senior Lecturer and Lister fellow (1998-2003) mitochondria biogenesis; chaperone-mediated and oxidative protein folding (University of Manchester, UK, work supported by Lister Fellowship, Wellcome Trust, BBSRC, MRC career establishment grant, Leverhulme Trust, Royal Society, EU). Military sevice (2000-2001). Assistant Professor and Group Leader (2003-2007) mitochondria biogenesis and cellular biochemistry (Univerisity of Crete, Chemistry Dept., and IMBB-FORTH). Associate Professor and Group Leader (2007- ) Mitochondria biogenesis in health and disease; protein folding; mitochondria nanomedicine (Dept. of Materials Science and Technology, Univ. of Crete and IMBB-FORTH, work funded by EU FP7, GSRT).

Selected Recent Publications

• Banci L., Bertini I., Cefaro C., Cenacchi, L., Ciofi-Baffoni S., Felli, I.C., Gallo A., Gonnelli, L., Luchinat, E., Sideris D.P., and Tokatlidis K. "A molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import", Proc Natl Acad. Sci USA 2010, (in press).
• Sideris, D.P., Petrakis, N., Katrakili, N., Mikropoulou, D., Gallo, A., Ciofi-Baffoni, S., Banci, L., Bertini, I., and Tokatlidis, K., "A novel targeting signal primes precursors for correct cysteine docking onto Mia40 in the mitochondrial intermembrane space", J. Cell Biol. 187(7), 1007-1022, 2009.
• Banci L., Bertini I., Cefaro C., Ciofi-Baffoni S., Gallo A., Martinelli M., Sideris DP, Katrakili N., and Tokatlidis K. "MIA40 is an oxidoreductase catalyzing oxidative protein folding in mitochondria", Nature Structural and Molecular Biology 16(2), 198-206, 2009.
• Lionaki, E., de Marcos Lousa, C., Baud, C., Vougioukalaki, M., Panayotou, G., and Tokatlidis, K, "The essential function of Tim12 in vivo is ensured by the assembly interactions of its C-terminal domain", J. Biol. Chem. 283(23), 15747-15753, 2008.
• Tokatlidis, K. "A disulfide relay system in mitochondria", Cell, 121, 965-967, 2005.
• Luciano, P., Vial S., Vergnolle M., Dyall S., Robinson D., and Tokatlidis K. "Functional reconstitution of the yeast ADP/ATP carrier import mediated by the TIM10 complex", EMBO J. 20(15) 4099-4106, 2001.
• Koehler, C.M., Merchant, S., Oppliger. W, Schmid, K., Jarosch, E., Dolfini, L., Junne, T., Schatz, G. and Tokatlidis, K. "Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins" EMBO J. 17, 6477-6486. 1998.
• Koehler, C.M., Jarosch, E., Tokatlidis, K., Schmid, K., Schweyen, R.J., and Schatz, G., "Import of mitochondrial carriers mediated by essential proteins of the intermembrane space", Science 279, 369-373 1998.
• Tokatlidis, K., Junne, T., Moes, S., Schatz, G., Glick, B.S., and Kronidou, N., "Translocation arrest of an intramitochondrial sorting signal next to Tim11p, a novel protein of the inner membrane import site". Nature 384, 585-588, 1996.