Non-linear microscopy techniques are at the forefront of biomedical research over the last decade. These non destructive modalities offer improved resolution, high contrast images with increased penetration depth and complementary information while minimizing phototoxicity and photodamage effects on the biological samples. These properties characterize them as perfect imaging tools for revealing valuable and unique information of the specimen under investigation. These techniques are not limited to visualization since they also permit precise quantitative analysis and testing of specific mechanisms and biological processes. In this talk, the imaging modalities of MPEF, SHG and THG will be presented as novel diagnostic tools for the in vivo sub-cellular investigation of complex biological activities and the extraction of structural and morphological information from various samples. Moreover, applications of non-linear imaging microscopy techniques to Cultural Heritage objects will be discussed.
For forthcoming colloquia, please see: http://www.materials.uoc.gr/el/colloquia